The Drosophila Hippo pathway transcription factor Scalloped and its co-factors alter each other's chromatin binding dynamics and transcription in vivo

SA Manning; B Kroeger; Q Deng; E Brooks; Y Fonseka; E Hinde; KF Harvey

Journal article

The Drosophila Hippo pathway transcription factor Scalloped and its co-factors alter each other's chromatin binding dynamics and transcription in vivo

SA Manning, B Kroeger, Q Deng, E Brooks, Y Fonseka, E Hinde, KF Harvey

Developmental Cell | CELL PRESS | Published : 2024

DOI: 10.1016/j.devcel.2024.04.006

Abstract

The Hippo pathway is an important regulator of organ growth and cell fate. The major mechanism by which Hippo is known to control transcription is by dictating the nucleo-cytoplasmic shuttling rate of Yorkie, a transcription co-activator, which promotes transcription with the DNA binding protein Scalloped. The nuclear biophysical behavior of Yorkie and Scalloped, and whether this is regulated by the Hippo pathway, remains unexplored. Using multiple live-imaging modalities on Drosophila tissues, we found that Scalloped interacts with DNA on a broad range of timescales, and enrichment of Scalloped at sites of active transcription is mediated by longer DNA dwell times. Further, Yorkie increased..

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University of Melbourne Researchers

Elizabeth Hinde Author

Kieran Harvey Author

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Grants

Awarded by Australian Cancer Research Foundation

Funding Acknowledgements

We thank members of the Harvey lab and Senthil Arumugam for discussions and comments on the manuscript and Kaustubh Wagh from the Hager lab for assistance with SMT analysis. We thank the Bloomington Drosophila Stock Center; the Vienna Drosophila RNAi Center; the Australian Drosophila Research Support Facility; L. Cheng, I. Hariharan, J. Jiang, and D. Pan for D. melanogaster stocks; and Xiaomeng Zhang for plasmids. K.F.H. was supported by a Senior Research Fellowship (APP1078220) and Investigator grant (APP1194467) from the National Health and Medical Research Council of Australia (NHMRC) . E.H. was supported by a Future Fellowship (FT200100401) from the Australian Research Council (ARC) . This research was supported by the ARC (DP180102044, DP190101743, DP22010523, and DP230101406) and the NHMRC (APP1157737) . We acknowledge the Monash Micro Imaging Facility. We acknowledge the Peter Mac Centre for Advanced Histology and Microscopy and support to them from the PeterMacCallum Cancer Foundation and the Australian Cancer Research Foundation.r MacCallum Cancer Foundation and the Australian Cancer Research Foundation.